Glycoside-metabolizing oxidoreductase D3dgpA from human gut bacterium

The Gfo/Idh/MocA family enzyme DgpA was known to catalyze the regiospecific oxidation of puerarin to 3”-oxo-puerarin in the presence of 3-oxo-glucose. Here, we discovered that D3dgpA, dgpA cloned from the human gut bacterium Dorea sp. MRG-IFC3, catalyzed the regiospecific oxidation of various C-/O-glycosides, including puerarin, in the presence of methyl β-D-3-oxo-glucopyranoside. While C-glycosides were converted to 3”- and 2”-oxo-products by D3dgpA, O-glycosides resulted in the formation of aglycones and hexose enediolone from the 3”-oxo-products. From DFT calculations, it was found that isomerization of 3”-oxo-puerarin to 2”-oxo-puerarin required a small activation energy of 9.86 kcal/mol, and the O-glycosidic bond cleavage of 3”-oxo-products was also thermodynamically favored with a small activation energy of 3.49 kcal/mol. In addition, the reaction mechanism of D3dgpA was discussed in comparison to those of Gfo/Idh/MocA and GMC family enzymes. The robust reactivity of D3dgpA was proposed as a new general route for derivatization of glycosides.


Introduction
C-Glycosides have been noticed as important bioactive compounds.Particularly, natural aryl C-glycosides are a group of natural products with the structural feature of an aromatic moiety and sugar(s) directly connected through a glycosidic C-C bond (Kitamura et al., 2018).Due to the various biological activities, such as antibacterial, antitumor, antidiabetic, and antioxidant activities (Xiao et al., 2016;Min et al., 2021;Tao et al., 2022;Xie et al., 2022), research on biosynthesis, chemical synthesis (Ni et al., 2022;Chong et al., 2023), and bioavailability (Peng et al., 2021) of C-glycosides has increased recently.One of the characteristics of C-glycosides is the inertness to biological metabolism.Only a few human gut bacteria have been reported to be able to degrade C-glycosides (Supplementary Table S1).Accordingly, the metabolism of C-glycosides in the human body has drawn a great deal of interest (Mori et al., 2021;Choi et al., 2023).
Puerarin is a major bioactive compound from the root of Pueraria lobata used in traditional medicine for the treatment of cardiovascular and cerebrovascular diseases, diabetes and diabetic complications, osteonecrosis, Parkinson's disease, Alzheimer's disease, endometriosis, and cancer (Zhou et al., 2014).It belongs to the isoflavone C-glycoside, and its stereospecific conversion to S-equol through the formation of daidzein in the human gut was elucidated (Kim et al., 2010;Park et al., 2011) (Figure 1).Gut metabolism of puerarin is known to begin with the regioselective oxidation of puerarin by NAD + -dependent oxidoreductase (Nakamura et al., 2020).Recently, 3"-oxidation of C-glycosides has been reported in environments such as soil and human gut, as a general biochemical metabolism of C-glycoside degradation (Kim et al., 2015;Kumano et al., 2021).However, how the regioselective oxidation of the C3 center of C-glycosides leads to the subsequent cleavage of the C-glycosidic bond is not wellunderstood yet.
Until now, two groups of oxidoreductases, GMC (glucose-methanol-choline) and Gfo/Idh/MocA families, have been reported to perform the regioselective oxidation of C-glycosides.Both families of enzymes, represented by CarA and DgpA, respectively, do not have sequence homology.While DgpA requires NAD + , CarA utilizes FAD for the oxidation of C-glycosides (Nakamura et al., 2020;Kumano et al., 2021).Accordingly, regeneration of the oxidized cofactors during catalysis is also different between these two oxidoreductases.In the case of DgpA, 3-oxo-glucose can oxidize NADH to regenerate NAD + .Molecular oxygen oxidizes FADH 2 of CarA with the formation of hydrogen peroxide (Figure 2).
Recently, the convergent evolution of GMC and Gfo/Idh/MocA enzymes for metabolizing carbohydrates and C-/O-glycosides was suggested independently (Liu et al., 2007;Kuritani et al., 2020).The hypothesis implies that regiospecific oxidation of the monosaccharide moiety of the substrates is an important biochemical reaction in the biological system, though its significance is not completely understood.In contrast, C-glycoside metabolism by these enzymes provides an opportunity for the development of new biocatalysts due to the potential biotechnological applications of the reaction products.For example, it would allow the derivation of the sugar moiety of C-glycoside-derived antibiotics.
Due to the growing significance of glycoside oxidases in green biotechnology, the gene responsible for the oxidation of puerarin was cloned from the newly isolated Dorea sp.MRG-IFC3 strain (Mi et al., 2023).By utilizing the heterologously expressed enzyme D3dgpA, we have designed a more convenient biocatalytic system and studied the reactivity of D3dgpA with various glycosides and the effect of other monosaccharides.From the study, we have discovered that D3dgpA exhibited O-glycosidase activity for various O-glycosides as well as regiospecific glycoside oxidation.Furthermore, it was found by means of computational chemistry that the O-glycosidic bond cleavage observed from the reaction of D3dpgA is a non-enzymatic reaction due to the instability of 3"-oxo-O-glycoside products.Isoflavone to S-equol metabolism in the human gut.Soy isoflavone daidzin is converted to daidzein by β-glucosidase, which is further reduced to (3S, 4R)-tetrahydrodaidzein through the (3S)-dihydrodaidzein. Puerarin was converted to daidzein through the formation of 3"-oxo-puerarin.

Cloning and over-expression of D3dpgA
A polymerase chain reaction (PCR) was used to clone D3dgpA (GenBank number: OR238368.1).The genomic DNA of Dorea sp.MRG-IFC3 (KCTC25707) was used as a template.Two primers, 5'-gaattcaATGAGTAAATTAAAAATTGGTATTATTG-3' (lower case letters are EcoRI site.) as a forward primer and 5'-gtcgac TTAGAATTTAATTGTCTCATTTGTTT-3' (lower case letters are SalI site) as a reverse primer were used.The PCR product was subcloned in pGEMT-easy vector (Promega) and sequenced.The D3dgpA was subcloned into the EcoRI/SalI site of the pET-duet 1 vector (Novagen).The resulting construct was transformed into Escherichia coli BL21 (DE3).To induce the protein, the overnight culture on LB plate was inoculated into LB broth containing 50 μg/ mL of ampicillin and incubated at 37 °C, 150 rpm until the OD at 600 nm reached 0.6.After adding IPTG to the final concentration of 0.5 mM, the culture was incubated at 18 °C for 16 h.The culture was harvested by centrifugation at 5,000 g for 25 min at room temperature (yield 5 g/L of wet cells).

Purification of D3dgpA and enzyme assay
The overexpressed D3dgpA was purified using Ni-NTA affinity column chromatography.The cell pellet was suspended in the lysis buffer (1.0 g/mL, Tris 50 mM, NaCl 500 mM, pH 8.0) and subjected to sonication (15 s pulse/30 s pause, 36 cycles at 4 °C).After centrifugation (25,000 g, 60 min, 4 °C) of the cell lysate, the collected supernatant was filtered through a cellulose acetate filter (0.45 μm) and loaded on the Ni-NTA resin (Novagen).After thorough washing with 20 mM imidazole (Tris 50 mM, NaCl 500 mM, pH 8.0), step elution was performed.D3dgpA was eluted at a concentration between 250 mM and 400 mM of imidazole.Each fraction's purity and quantification were measured by SDS-PAGE (Tris-glycine) and Bradford assay, respectively.Elution fractions were concentrated and dialyzed with MWCO 10KD UF (Spin-X ® UF 20).After purification, the quantity of DgpA was determined by UV absorption at 280 nm or Bradford assay (595 nm).
All the enzyme reactions, including the study of enzyme kinetics and the effects of additives, were performed multiple times to check their reproducibility.A typical enzyme assay was performed with 700 equivalents of puerarin (10 mM in DMF) and methyl β-D-3oxo-glucopyranoside (40 mM in water) with respect to D3dgpA in Tris buffer (10 mM, NaCl 10 mM, pH 8.0) at 37 °C with stirring (250 rpm).The reaction was initiated by adding D3dgpA and stopped by adding ethyl acetate.The reaction product was extracted with ethyl acetate from twice the volume of the reaction mixture, which was dried under vacuum.The dried residue was dissolved in methanol.The methanolic solution was filtered with a syringe filter (0.22 μm, PTFE) before HPLC analysis.The reaction product of the assay was analyzed by Dionex Ultimate 3000 UHPLC with Kinetex ® C18 column (1.7 μm, Phenomenex).
The mobile phase was as follows: A, H 2 O with 0.1% acetic acid and B, acetonitrile.Linear gradient elution was adopted (5% of B at 0 min and increased to 55% at 15 min).

Activity of D3dgpA with alternative substrates
The reactivity of D3dgpA was monitored with various C-/ O-Glycoside substrates (10 mM in DMF).To initiate the reaction, D3dgpA (10 μM) was added to the solution containing 1 mM of the glycoside substrate and methyl β-D-3-oxoglucopyranoside in Tris buffer (10 mM, pH 8.0) at 37 °C.The reaction mixture (100 μL) was stirred (250 rpm) for 30 min.The reaction was stopped by adding 200 μL of methanol, and the reaction product was filtered through the syringe filter (0.22 μm, PTFE) before HPLC analysis.The flow rate was 0.2 mL/min, the column temperature was 35 °C, and the eluents of 1.0% formic acid and acetonitrile were used.

Analysis of the reaction product of methyl β-D-3-oxo-glucopyranoside
To confirm the function of β-D-3-oxo-glucopyranoside as an oxidant in the catalysis, the reaction product was analyzed after the enzyme reaction.The enzyme reaction mixture was filtered through a syringe filter and directly analyzed by LC/MS.UHPLC equipped with a Waters ACQUITY column (UPLC BEH C18 1.7 µm, 2.1 * 150 mm) was used for LC/MS analysis.The mobile phase comprising water 0.1% formic acid (v/v) (A) and acetonitrile 0.1% FA (v/v) (B) was used to apply the gradient system of B 1% (0 min), B 60% (6 min), and B 100% (7-8 min).The total run time was 10 min, and the flow rate was 0.4 mL/min at 50 °C.A mass spectrometer equipped with an Orbitrap detector was used.Negative-mode MS in the range of 120-1000 m/z was applied for the analysis of carbohydrates.

Computational study
Computational chemistry was performed by Gaussian 16 packages combined with GaussView 6 (Gaussian, Inc.CT, United States).The bioenergetics of regiospecific puerarin oxidation by D3dgpA with 3-oxo-glucopyranose or methyl β-D-3-oxo-glucopyranoside were studied by comparing Gibbs free energy for each reaction in water.In detail, the most stable conformers of puerarin, 3"-oxo-puerarin, methyl β-D-3-oxoglucopyranoside, β-D-3-oxo-glucopyranose, methyl β-Dglucopyranoside, and β-D-glucopyranose were found from the relaxed scan of dihedral angles.The free energy of the optimized structures was obtained at the B3BLYP level with the 6-311++G(d,p) basis set, including empirical dispersion and solvent effect.
The isomerization of 3"-oxo-puerarin to 2"-oxo-puerarin was investigated using the deprotonated anionic forms of both compounds.A transition state complex was obtained from the optimized compounds using the QST2 option.For the O-glycosidic bond cleavage of 3"-oxo-daidzin, a hydroxide ion was introduced, and the free energy of the optimized structure was compared with that of the 2"C-deprotonated 3"-oxo-daidzin and water.

Characteristics of D3dgpA
The D3dgpA gene cloned from Dorea sp.MRG-IFC3 was of the same length as dgpA (Nakamura et al., 2020), but different in four nucleotides (OR238368.1).However, the primary structure of D3dgpA, comprising 367 amino acid residues, was identical to that of DgpA of strain PUE (BBG22493.1,Supplementary Figure S4).D3dgpA with His 6 -tag was purified by Ni-NTA affinity column chromatography and centrifugal filtration desalting.The estimated molecular weight of D3dgpA was 45 kD, and it seemed to form a hexamer in solution as reported (Supplementary Figure S5) (He et al., 2023).The catalysis by D3dgpA is known to follow the pingpong mechanism.For example, the first reaction step produces 3"oxo-glycoside and bound NADH, and the second reaction step reduces 3-oxo-glucose to regenerate NAD + (Nakamura et al., 2020).In this study, the activity of D3dgpA was monitored with methyl β-D-3-oxo-glucopyranoside, instead of 3-oxo-glucose, because it is easy to prepare and not isomerized to the other isomers in solution.Like 3-oxo-glucose, methyl β-D-3-oxo-glucopyranoside acted as an oxidant to generate NAD + during the catalysis.The reduced product, methyl β-D-glucopyranoside, was identified from the reaction mixture by LC-MS analysis (Supplementary Figure S6).However, other oxidants, such as glucuronolactone or αketoglutarate, reported to regenerate NAD + in the same family of oxidoreductases (Taberman, et al., 2016), were not able to reconstitute the catalysis of D3dgpA.
The purified D3dgpA was found to contain an almost equimolar amount of NAD(H), and the ratio of NADH to NAD + was determined to be 3 to 7 by HPLC analysis (Supplementary Figure S7).When additional amounts of NAD + were introduced to the reaction mixture, NAD + inhibited the activity of D3dgpA.Thus, allosteric regulation by NAD + was suspected (Supplementary Figure S8).
D3dgpA showed the highest activity at pH 8.0 and exhibited high stability at room temperature (Supplementary Figures S9, S10).Approximately 80% of activity was maintained for 3 weeks.Although it was reported that manganese ions were required for the activity of D3dgpA in an earlier report (Nakamura et al., 2013), it was confirmed that D3dgpA does not require manganese or any other divalent metal ions, as evident from EDTA treatment.
Because D3dgpA reacted with various glycosides by acting on the glycosyl group, the effect of other monosaccharides on puerarin oxidation was investigated to evaluate whether they interfered with puerarin oxidation by D3dgpA (Figure 5).Among the tested monosaccharides, D-glucose exhibited the most inhibitory effects on the oxidation of puerarin in the presence of methyl β-D-3-oxoglucopyranoside.The inhibitory effect of monosaccharides decreased in the order of D-glucose > methyl ß-glucoside > methyl α-glucoside ≈ levoglucosan.Arabinose and gulose appeared to potentiate the activity of D3dgpA for the oxidation of puerarin.The inhibition by D-glucose emphasizes high substrate specificity for glucosides and can be considered a product inhibition since D3dgpA catalyzes an equilibrium reaction vide infra (Choi et al., 2023).The results can also be applied to the biotechnological application of Dorea sp.MRG-IFC3 when the medium composition is optimized.

Bioenergetics and reaction mechanism of D3dgpA catalysis
One of the rationales for the investigation of biochemical metabolism is bioenergetics of the enzyme-catalyzed reaction.With 3-oxo-glucose, the reaction of 3"-oxo-puerarin formation catalyzed by D3dgpA was found to have an equilibrium constant close to 1 (Eq. 1) (Choi et al., 2023).Since methyl β-D-3-oxoglucopyranoside was used for the study of D3dgpA, we have first compared the free energy change of the D3dgpA-catalyzed reaction in the presence of methyl β-D-3-oxo-glucopyranoside (Eq.2) by means of DFT computational chemistry at the level of G6-311++(d,p).The reaction was found to be endergonic by 0.27 kcal/mol under the same conditions.Although the reaction became slightly more endergonic than the reaction utilizing 3-oxoglucose by 0.09 kcal/mol, the equilibrium constant was practically the same as shown in Eq. 2. Therefore, methyl β-D-3-oxoglucopyranoside was found to be a suitable alternative oxidant for D3dgpA from the perspective of bioenergetics.(2) Likewise, D3dgpA-catalyzed oxidation of daidzin was found to be slightly endergonic (Eq.3).The formation of 3"-oxo-daidzin by D3dgpA appeared to be unfavorable thermodynamically, by 0.70 kcal/mol, when compared to the formation of 3"-oxopuerarin (Eq.1).It could be one of the reasons why D3dgpA is more reactive against puerarin than daidzin (Supplementary Figure S12).daidzin + 3 − oxo − glucose ↔ 3" − oxo − daidzin + glucose; 0.88 kcal mol. (3) Next, O-glycosidic cleavage reaction of 3"-oxo-O-glycosides was investigated with daidzin.After the formation of 3"-oxo-daidzin by D3dgpA, the product was converted to daidzein and hexose enediolone.Previously, the cleavage of O-glycosidic bond of 3"oxo-O-glycosides was proposed as a non-enzymatic reaction (Kumano et al., 2021).To check the proposal, we have investigated the thermochemistry of the reaction.Whereas the C-glycosidic bond cleavage of 3"-oxo-puerarin is slightly endergonic (Eq.4), the conversion of 3"-oxo-daidzin to daidzein and hexose enediolone was highly exergonic, by 10.13 kcal/mol (Eq.5).Therefore, it was found that the cleavage of 3"-oxo-daidzin O-glycosidic bond was favored thermodynamically.To our surprise, 3"-oxo-daidzin was converted to daidzein and hexose enediolone during the geometry optimization, when the hydroxide ion was introduced.Thus, the reaction appears to happen spontaneously in the presence of a hydroxide ion.
The activation energy of the isomerization between 3"-oxopuerarin and 2"-oxo-puerarin was also calculated by transition state search (Figure 6).The 2"C-deprotonated species of 3"-oxopuerarin (A) and the 3"C-deprotonated species of 2"-oxo-puerarin (B) were almost the same in free energy.Enediolate anion species (TS) was found as a transition state complex, whose free energy was higher than that of the 3"-oxo-puerarin anion (A) only by 9.86 kcal/ mol.Therefore, the isomerization of 3"-oxo-C-glycoside to 2"-oxo-C-glycoside was found feasible thermodynamically.
The reaction catalyzed by D3dgpA is reminiscent of other Gfo/ Idh/MocA-like oxidoreductases, which also utilize NAD(P) + to form glycoside oxo-products.However, D3dgpA from Dorea sp.MRG-IFC3 is unique, in that it reacts with C-glycosides and produces 3"oxo-products instead of the hydrolyzed products.D3dgpA appears to follow the same reaction mechanism as other enzymes utilizing NAD + for the biochemical oxidation of 3"C-OH of the glycosyl group (Figure 7).However, other oxidoreductases go through the subsequent deprotonation of the acidic 2"C-H by the tyrosyl anion residue, which facilitates the cleavage of C-O-glycosidic bond to release the aglycone (Figure 7B).Then, the water is added to the hexose enediolone intermediate to yield the enolate intermediate (Figure 7C).The second intermediate is protonated by the tyrosyl residue to produce 3-oxo-glucose (Figure 7D).The third intermediate, 3-oxo-glucose, is then reduced to glucose by NADH, to regenerate NAD + (Figure 7E).Finally, the glucose is exchanged with the new glycoside for the next cycle of reaction (Figure 7F).In the case of D3dgpA, the catalysis cannot afford the cleavage of the C-glycosidic bond of 3"-oxo-C-glycoside even after 2"C-H deprotonation (Figure 7B).Thus, it should release 3"-oxo-C-glycoside from the catalytic site and accept 3-oxo-glucose to regenerate NADH (Figure 7E).Namely, it catalyzes the reaction steps of A → B → E → F → A in Figure 7. Compared to other Gfo/ Idh/MocA and GMC family enzymes, D3dgpA only catalyzed regioselective oxidation of C-/O-glycosides and did not undergo further hydration and regeneration of cofactors (Figure 7).A recent report on the GMC family enzyme, GlycDH, also emphasized that the substrate specificity and C-/O-glycosidic bond cleavage of the related enzymes cannot be explained by the active site structure or the Mn 2+ cofactor (Bitter et al., 2023).

Discussion
In this report, we have studied the characteristics and reactivity of D3dgpA, a Gfo/Idh/MocA family glycoside oxidoreductase.Expression of D3dgpA in Dorea sp.MRG-IFC3 was induced by puerarin (Mi et al., 2023), and it appeared to form a hexameric quaternary structure.Though D3dgpA exhibited higher reactivity for C-glycosides than O-glycosides, it also efficiently catalyzed the oxidation of O-glycosides (Table 1, Figure 4).One of the reasons we specifically investigated the activity of D3dgpA was the high substrate specificity of Dorea sp.MRG-IFC3 in the cleavage of the C-glycosidic bond.The IFC3 strain only metabolized puerarin, but not the other C-glycosides.
From the reactivity study of D3dgpA, it is now clear that puerarinspecific C-deglycosylation is due to the C-deglycosidase, D3dgpBC, which metabolizes 3"-oxo-puerarin (Figure 1).
D3dgpA catalyzed the oxidation of glycosides, resulting in more than a single product, but it is believed that the direct product formed from the catalysis is 3"-oxo-glycosides.In the case of C-glycosides, 3"-oxo-C-glycosides were isomerized to 2"-oxo-C-glycosides through keto-enol tautomerization.The ratio of 3"oxo-to 2"-oxo-C-glycoside was different depending on C-glycoside products because of the thermodynamic stability of the isomers produced by the non-enzymatic reaction.From the computational chemistry approach, it appeared that the activation energy of the isomerization between 3"-oxo and 2"-oxo-C-glycosides was low enough to occur non-enzymatically.In addition, 3"-oxo-O-glycosides products were further converted to 2"-oxo-O-glycosides and cleavage products of the O-glycosidic bond, aglycone and hexose enediolone (Figure 7D), through the nonenzymatic reaction.Therefore, 2"-oxo-C-glycosides, 2"-oxo-O-glycosides, aglycones, and hexose enediolone are the products formed by the non-enzymatic reactions due to the stability of 3"oxo-glycosides.The results also imply a significant energy barrier for cleavage of the C-glycosidic bond of 3"-oxo-glycosides, which cannot be overcome with a Gfo/Idh/MocA family glycoside oxidoreductase.The cleavage of the C-glycosidic bond of 3"-oxo-C-glycosides is catalyzed only by C-deglycosidase (Mori et al., 2021;Choi et al., 2023).Proposed pathway for the isomerization of 3"-oxo-puerarin to 2"-oxo-puerarin (B).Intramolecular hydrogen transfer between two deprotonated 3"-oxo-puerarin anion (A) and 2"-oxo-puerarin anion (B) had a transition state complex (TS) in a higher energy state by 9.86 kcal/mol.
Along with the structural features, the similarity of the proposed mechanism between O-and C-glycosidic bond cleavages observed from 3"-oxo-glycosides still puzzles scientists as to why the C-glycosidic bond cleavage is feasible only by C-deglycosidase (Bitter et al., 2023).The Mn 2+ ion in the active site of C-deglycosidase and/or the α-OH group of the aglycone adjacent to the 3"-oxo-glucosyl unit of the substrate is not sufficient to guarantee the cleavage of the C-glycosidic bond.Otherwise, glycoside hydrolase family 4 enzymes, belonging to the Gfo/Idh/ MocA family, could cleave the C-glycosidic bond of 3"-oxo-C-glycosides (Varrot et al., 2005).As found from our computational study, 3"-oxo-C-glycosides are much more stable than 3"-oxo-O-glycosides.Therefore, it appears the other catalytic mechanisms are required to lower the activation energy of the C-glycosidic bond cleavage and the Mn 2+ ion in the active site and structural features of the substrate.
Whereas a plethora of glycoside-metabolizing biochemical reactions, including lyase, hydrolase, and transferase, are available, redox-active cofactor-dependent glycoside oxidoreductases, such as Gfo/Idh/MocA and GMC family enzymes, have emerged as a new group of glycoside-metabolizing enzymes (Bitter et al., 2023;Taborda et al., 2023).These glycoside oxidoreductases require NAD(P) + or flavin for the oxidation of glycosides.Whereas flavin-utilizing enzymes utilize molecular oxygen and other abiological oxidants, such as 1,4-benzoquinone, for the catalysis (Figure 2), NAD(P) + -dependent enzymes regenerate The reaction mechanism of D3dgpA in comparison to other glycoside oxidoreductases.D3dgpA-catalyzed reaction pathway is shown in the blue panel.General base deprotonates the 3"O-H and the 3"-hydride is subsequently transferred to NAD + (A).In the case of other glycoside hydrolases, tyrosyl residue in the active site deprotonates the 2"-H to break the O-glycosidic bond with the formation of hexose enediolone (B).A water molecule is added on the β-position of hexose enediolone (C) and protonation of C3 position is achieved by the tyrosyl residue to form the 3-oxo-glucose (D).However, D3dgpA releases the 3"-oxo-product and binds 3-oxo-glucose as the second substrate, to regenerate NAD + (E).With the formation of NAD + , the formed glucose molecule leaves the active site and the next glycoside binds to initiate the next catalytic cycle (F).
the oxidant cofactor by reducing other metabolites, such as 3-oxoglucose and α-ketoglutarate.The emerging mechanism of glycoside catabolism appears to involve the formation of 3"-oxo-glycosides, but their utilization in biochemistry is not limited to the degradation of glycosides to aglycones.These enzymes can also be used for biotechnological applications to provide new bioactive C-glycosides.Especially, D3dgpA, stable at room temperature for 3 weeks, appears to have by far the largest substrate binding site among the reported Gfo/Idh/MocA family enzymes (Yip et al., 2004;Varrot et al., 2005;Thoden and Holden, 2010;Thoden and Holden, 2011;Mukherjee et al., 2019;Kuritani et al., 2020;Yoshiwara et al., 2020;Kumano et al., 2021).Hence, D3dgpA can be used for the regioselective functionalization of the known bioactive C-glycosides through the 3"-oxo-C-glycosides (Kitamura et al., 2018).Often, regioselective chemical functional group transformation using carbohydrates is very difficult, but D3dgpA provides the new enzyme synthesis.

Conclusion
In this report, we have investigated the reactivity of D3dgpA, cloned from Dorea sp.MRG-IFC3.D3dgpA has exhibited a broad substrate spectrum for C-/O-glycosides.Regiospecific oxidation of the glucosyl unit of the glycosides resulted in the formation of 3"oxo-C-/O-glycosides.The formation of 2"-oxo-C-glycosides was achieved by non-enzymatic isomerization in the reaction mixture.In addition, 3"-oxo-O-glycosides were also converted to aglycones abiologically due to the low transition state and instability of 3"-oxo-O-glycosides.Our study has made D3dgpA a potentially important enzyme for valorizing natural glycosides.

FIGURE 2
FIGURE 2 Cofactor regeneration by DgpA and CarA during the catalysis of C-glycoside oxidation.